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Maligeppagol, Manamohan
- Development of Recombinant Cry3A Expressing Bacillus cereus Isolated from the Rhizosphere of Brinjal (Solanum melongena L.) in the Management of Ash Weevil (Myllocerus subfasciatus Guerin)
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Authors
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake PO., Bangalore - 560 089, Karnataka, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake PO., Bangalore - 560 089, Karnataka, IN
Source
Journal of Biological Control, Vol 26, No 2 (2012), Pagination: 44-49Abstract
A predominant Bacillus cereus (BR145) was engineered to express Cry3A protein by electroporation from B. thuringiensis tenebrionis (Btt.) The rhizospheric survival of these Bacillus spp., was compared where survival of the former was better in the rhizosphere of brinjal than the latter. The efficacy of Cry3A protein obtained from the lysed cells of rBR145 and Btt were evaluated for toxicity against grubs and adult M. subfasciatus in pot and detached leaf bioassays, respectively. The toxicity of Cry3A protein produced from both the bacilli was on par.Keywords
Cry3A, Myllocerus subfasciatus, Bacillus cereus, Bacillus thuringiensis Ssp. Tenebrionis and Rhizosphere.Full Text
References
- Bimboim HC, Doly J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7: 1513–1523.
- Casique-Arroyo G, Bideshi D, Salcedo-Herna´ndez R, Barboza-Corona JE. 2007. Development of a recombinant strain of Bacillus thuringiensis subsp. kurstaki HD-73 that produces the endochitinase ChiA74. Antonie van Leeuwenhoek, 92: 1–9.
- Cerón J, Ortíz A, Quintero R, Güereca L, Bravo A. 1995. Specific PCR primers directed to identify cryI and cryIII genes within a Bacillus thuringiensis strain collection. App Env Microbiol. 61: 3826–3831.
- Gowda KS, Veeresh GK. 1984. Assessment of crop loss in brinjal (Solanum melongena L.) due to as weevil Myllocerus subfasciatus Guerin. (Curculionidae : Coleoptera). J Soil Biol Ecol. 4: 41–52.
- Granum PE, O’Sullivan K, Lund T. 1999. The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus. FEMS Microbiol Letters 177:225–229.
- Knight JS, Broadwella AH, Grant WN, Shoemaker CB. 2004. A strategy for shuffling numerous Bacillus thuringiensis crystal protein domains. J Econ Ent. 97: 1805–1813.
- Milus EA, Rothrock CS. 1993. Rhizosphere colonization of wheat by selected soil bacteria over diverse environments. Canadian J Microbiol. 39: 335–341.
- Oscariz JC, Lasa I, Pisabarro AG. 1999. Detection and characterization of cerein 7, a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity. FEMS Microbiol Letters, 178: 337–341.
- Palm CJ, Schaller DL, Donegan KK, Seidler RJ. 1996. Persistence in soil of transgenic plant-produced Bacillus thuringiensis var. kurstaki ä-endotoxin. Canadian J Microbiol. 42: 1258–1262.
- Raymond B, Wyres KL, Sheppard SK, Ellis RJ, Bonsall MB. 2010. Environmental factors determining the epidemiology and population genetic structure of the Bacillus cereus group in the field. PLoS Pathogens, 6: e1000905. doi:10.1371/journal.ppat.1000905
- Saade FE, Dunphy GB, Berlier RL. 1996. Response of the carrot weevil, Listronotus oregonensis (Coleoptera: Cucrulionidae), to strains of Bacillus thuringiensis. Biol Control 7: 293–298.
- Selinger, LB, Kachatourians GG, Byers JR, Hynes MF. 1998. Expression of a Bacillus thuringiensis ä-endotoxin gene by Bacillus pumillus. Canadian J Microbiol. 44: 259–269.
- Sudha SN, Jayakumar R, Vaithilingam S. 1999. Introduction and expression of the cry1Ac gene of Bacillus thuringiensis in a cereal-associated bacterium, Bacillus polymyxa. Curr Microbiol. 38: 163–167.
- Vijayalakshmi K, Suseela R. 2010. Antimicrobial protein production by Bacillus amyloliquefaciens MBL27: An application of statistical optimization technique. African J Microbiol Res. 4: 2388–2396.
- Wang G, Zhang J, Song FWJ, Feng S, Huang D. 2006.
- Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Appl Microbiol Biotechnol. 72: 924–930.
- Weller DM. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annl Rev Phytopathol. 26: 379–407.